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Table 4 Primers used for real-time gene expression analysis. The amplicon size generated by each primer pair is shown.

From: Molecular characterization and expression analysis of five different elongation factor 1 alpha genes in the flatfish Senegalese sole (Solea senegalensis Kaup): Differential gene expression and thyroid hormones dependence during metamorphosis

Target Primers   Fragment size (bp)
  Primer pair name Sequence  
SseEF1A1 SseEF1•1 5'-GATTGACCGTCGTTCTGGCAAGAAGC-3' (F) 142
  SseEF1•2 5'-GGCAAAGCGACCAAGGGGAGCAT-3' (R)  
SeeEF1A2 SseEF2•1 5'-TGGAAATGTGGCCGGTGACAGCA-3' (F) 124
  SseEF2•2 5'-GCAATCCAGAACGGGGGCGTA-3' (R)  
SseEF1A3 SseEF3•1 5'-GACCGTCGTAGTGGTAAAGCCCTGGAA-3' (F) 122
  SseEF3•2 5'-GGGGGAAACTCTGCAAACGCCTCA-3' (R)  
SseEF1A4 SseEF4•1 5'-GCCGTCGGTGTCATCAAGAAAGTCG-3' (F) 135
  SseEF4•2 5'-GAGATAAATCCTAATGTGGCTCCGTTGTCG-3' (R)  
Sse42Sp50 Sse42Sp50•1 5'-CCGGCTACTCCCCTGTGCTGGACT-3' (F) 62
  Sse42Sp50•2 5'-CAGCTCGGCAAAGCGGCAGGT-3' (R)