Table 4 Primers used for real-time gene expression analysis. The amplicon size generated by each primer pair is shown.
Target | Primers | Â | Fragment size (bp) |
|---|---|---|---|
| Â | Primer pair name | Sequence | Â |
SseEF1A1 | SseEF1•1 | 5'-GATTGACCGTCGTTCTGGCAAGAAGC-3' (F) | 142 |
|  | SseEF1•2 | 5'-GGCAAAGCGACCAAGGGGAGCAT-3' (R) |  |
SeeEF1A2 | SseEF2•1 | 5'-TGGAAATGTGGCCGGTGACAGCA-3' (F) | 124 |
|  | SseEF2•2 | 5'-GCAATCCAGAACGGGGGCGTA-3' (R) |  |
SseEF1A3 | SseEF3•1 | 5'-GACCGTCGTAGTGGTAAAGCCCTGGAA-3' (F) | 122 |
|  | SseEF3•2 | 5'-GGGGGAAACTCTGCAAACGCCTCA-3' (R) |  |
SseEF1A4 | SseEF4•1 | 5'-GCCGTCGGTGTCATCAAGAAAGTCG-3' (F) | 135 |
|  | SseEF4•2 | 5'-GAGATAAATCCTAATGTGGCTCCGTTGTCG-3' (R) |  |
Sse42Sp50 | Sse42Sp50•1 | 5'-CCGGCTACTCCCCTGTGCTGGACT-3' (F) | 62 |
|  | Sse42Sp50•2 | 5'-CAGCTCGGCAAAGCGGCAGGT-3' (R) |  |