Relative SseEF1A4 expression levels as determined by real-time quantitative PCR in the untreated control (grey), and in TU (black) and TU+T4 (white) treated groups. Larvae samples were collected for rRNA isolation at 8 and 13 days after T4 treatment starting 7 DPH. Expression values were normalized to those of GAPDH2. Data are expressed as the mean fold change (mean ± SEM, n = 3) from the calibrator group (untreated control day 8). Values marked with an asterisk are significantly different (P < 0.05 or less) from the corresponding untreated control group values.