Table 3 Cultured head kidney cells: ranking of the reference gene candidate genes in all samples and within infection treatments, respectively.
all (n = 27) | Infected/Infected (n = 9) | Control/Infected (n = 12) | Infected/Control (n = 2) | Control/Control (n = 4) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Reference gene | Nf | gN | BK | Nf | gN | BK | Nf | gN | BK | Nf | gN | BK | Nf | gN | BK |
rpl13a | 1 | 1 | 3 | 5 | 3 | 1 | 3 | 3 | 5 | not determined | 6 | 1 | 4 | ||
gapd | 2 | 6 | 1 | 6 | 4 | 5 | 1 | 1 | 2 | 2 | 7 | 2 | |||
actb | 3 | 5 | 7 | 4 | 6 | 7 | 8 | 8 | 8 | 4 | 1 | 3 | |||
ubc | 4 | 1 | 6 | 8 | 7 | 2 | 4 | 4 | 6 | 1 | 6 | 1 | |||
b2m | 5 | 4 | 8 | 1 | 1 | 4 | 2 | 1 | 4 | 5 | 5 | 7 | |||
hprt1 | 6 | 7 | 5 | 9 | 9 | 9 | 7 | 7 | 1 | 8 | 3 | 6 | |||
taf2 | 7 | 8 | 2 | 7 | 8 | 8 | 6 | 6 | 3 | 7 | 8 | 8 | |||
eef1a | 8 | 3 | 9 | 3 | 1 | 3 | 5 | 5 | 9 | 3 | 4 | 5 | |||
rpo2 | 9 | 9 | 4 | 2 | 5 | 6 | 9 | 9 | 7 | 9 | 9 | 9 | |||