Table 2 Cultured spleen cells: ranking of the reference gene candidate genes in all samples and within infection treatments, respectively.
all (n = 21) | Infected/Infected (n = 5) | Control/Infected (n = 12) | Infected/Control (n = 2) | Control/Control (n = 2) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Reference gene | Nf | gN | BK | Nf | gN | BK | Nf | gN | BK | Nf | gN | BK | Nf | gN | BK |
gapd | 1 | 1 | 1 | 1 | 1 | 4 | 1 | 1 | 1 | not determined | not determined | ||||
ubc | 2 | 4 | 4 | 6 | 6 | 7 | 3 | 6 | 4 | ||||||
rpl13a | 3 | 3 | 3 | 2 | 1 | 3 | 5 | 5 | 3 | ||||||
actb | 4 | 1 | 2 | 3 | 5 | 6 | 2 | 3 | 5 | ||||||
rpo2 | 5 | 5 | 5 | 9 | 9 | 8 | 4 | 1 | 2 | ||||||
b2m | 6 | 6 | 6 | 7 | 7 | 2 | 7 | 7 | 7 | ||||||
eef1a | 7 | 7 | 8 | 4 | 3 | 1 | 8 | 8 | 8 | ||||||
taf2 | 8 | 8 | 9 | 8 | 8 | 9 | 6 | 4 | 6 | ||||||
hprt1 | 9 | 9 | 7 | 5 | 4 | 5 | 9 | 9 | 9 | ||||||