Table 2 Real-time quantitative PCR characteristics
Gene | Accesion No. | Forward primer | Reverse primer | Annealing °C | MgCl2 mM | Amplicon size (bp) |
|---|---|---|---|---|---|---|
HMBS | NM_000190 | ggcaatgcggctgcaa | gggtacccacgcgaatcac | 56 | 2.5 | 64 |
TBP | NM_003194 | tgcacaggagccaagagtgaa | cacatcacagctccccacca | 56 | 2.5 | 132 |
B2M | NM_004048 | atgagtatgcctgccgtgtga | ggcatcttcaaacctccatg | 56 | 2.5 | 101 |
ACTB | NM_001101 | ggcatcctcaccctgaagta | ggggtgttgaaggtctcaaa | 56 | 2.5 | 203 |
HPRT1 | NM_000194 | tgctcgagatgtgatgaagg | tcccctgttgactggtcatt | 56 | 2.5 | 192 |
RPL13A | NM_012423 | aaaaagcggatggtggttc | cttccggtagtggatcttgg | 56 | 4.5 | 168 |
SDHA | NM_004168 | tggacctggttgtctttggt | agtcgcagttccgatgttct | 56 | 3.5 | 166 |
UBC | NM_021009 | atcgctgtgatcgtcacttg | tccagcaaagatcagcctct | 64 | 3.5 | 164 |
18S | NM_022551 | atccctgaaaagttccagca | ccctcttggtgaggtcaatg | 56 | 2.5 | 186 |
GAPDH | NM_002046 | gagccacatcgctcagacac | catgtagttgaggtcaatgaagg | 60 | 3.5 | 150 |