Table 2 Oligonucleotides
From: Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate
Primer | Sequence |
|---|---|
Sac1(-500)fwd | TTGCGGCCGC ACAGCTACCACATCCCTGAC |
Sac1(-242)fwd | TTGCGGCCGC CCAAGCCTCGCTCCTATTGT |
Sac1(-170)fwd | TTGCGGCCGC CTGCACTACTGCTTACCCACA |
Sac1(-125)fwd | TTGCGGCCGC GAAGTTGAAAAGGCAAGGGA |
Sac1(-114)fwd | TTGCGGCCGC GGCAAGGGAAAAATACCACA |
Sac1(-100)fwd | TTGCGGCCGC ACCACAGGTTTAGATAAGGA |
Sac1(-83)fwd | TTGCGGCCGC GGAAATAGGAGAAAGGATTAG |
Sac1(-1)rev | GGCTCGAG ATCTAGACGAGAAAATATACG |
Sac1(-150)rev | GGCTCGAG TGTGGGTAAGCAGTAGTGCAG |
Sac1Δ(-100/-84)fwd | GAAAAGGCAAGGGAAAAATGGAAATAGGAGAAAGG |
Sac1Δ(-100/-84)rev | CCTTTCTCCTATTTCCATTTTTCCCTTGCCTTTTC |
Sac1Δ(-83/-70)fwd | CCACAGGTTTAGATAAGGATTAGAAACCATATCC |
Sac1Δ(-83/-70)rev | GGATATGGTTTCTAATCCTTATCTAAACCTGTGG |
Sac1Δ(-91/-84)fwd | GGGAAAAATACCACAGGTGGAAATAGGAGAAAGG |
Sac1Δ(-91/-84)rev | CCTTTCTCCTATTTCCACCTGTGGTATTTTTCCC |
Sac1Δ(-100/-92)fwd | GGCAAGGGAAAAATATTAGATAAGGAAATAGG |
Sac1Δ(-100/-92)rev | CCTATTTCCTTATCTAATATTTTTCCCTTGCC |
Opi1KOfwd | CATATCAGGCCAGAACGTGGCATTTTGTTTACAGTGCTGAAGATTGTACTGCTGAGAGTGCAC |
Opi1KOrev | AACTATATTATTCCGTATAATATTATTACTGGTGGTAATGCTGTGCGGTATTTCACACCG |