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Table 1 Plasmids and yeast strains

From: Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate

  Genotpye Origin
Plasmid   
   pGK25 CEN ARS URA3 GFP [24]
   pGK26 CEN ARS URA3 SAC1 5' UTR (-500/-1)-GFP [24]
   pAK29 CEN ARS URA3 SAC1 5' UTR (-242/-1)-GFP This study
   pAK30 CEN ARS URA3 SAC1 5' UTR (-170/-1)-GFP This study
   pAK34 CEN ARS URA3 SAC1 5' UTR (-500/-150)-GFP This study
   pAK36 CEN ARS URA3 SAC1 5' UTR (-125/-1)-GFP This study
   pAK38 CEN ARS URA3 SAC1 5' UTR (-83/-1)-GFP This study
   PAK40 CEN ARS URA3 SAC1 5' UTR (-114/-1)-GFP This study
   pAK42 CEN ARS URA3 SAC1 5' UTR (-100/-1)-GFP This study
   pAK47 CEN ARS URA3 SAC1 5' UTR Δ(-100/-84)-GFP This study
   pAK48 CEN ARS URA3 SAC1 5' UTR Δ(-83/-70)-GFP This study
   pAK49 CEN ARS URA3 SAC1 5' UTR Δ(-91/-84)-GFP This study
   pAK50 CEN ARS URA3 SAC1 5' UTR Δ(-100/-92)-GFP This study
Strain   
   ATY201 MATαtrp1-delta901 leu2-3,112 his3-delta200 ura3-52 lys2-801 suc2-delta9 can1::hisG [36]
   STY39 MATa trp1-delta901 leu2-3,112 his3-delta200 ura3-52 lys2-801 suc2-delta9 can1::hisG sac1::TRP [8]
   STY40 MATa leu2-3, 112 ura3-52 his3-delta200 trp1-delta901 lys2-801 suc2-delta9 sac1::TRP1 stt4::HIS3 pSTT4-4 (LEU2 CEN6 stt4-4) [8]
   STY47 Pik1::ADE2-1 sac1::TRP YEplac181::pik1-12 [8]
   PMY434 MATαtrp1-delta901 leu2-3,112 his3-delta200 ura3-52 lys2-801 suc2-delta9 can1::hisG opi1::HIS3 This study
   PMY435 MATa trp1-delta901 leu2-3,112 his3-delta200 ura3-52 lys2-801 suc2-delta9 can1::hisG sac1::TRP opi1::HIS3 This study