Table 1 Plasmids and yeast strains
From: Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate
Genotpye | Origin | |
|---|---|---|
Plasmid | ||
pGK25 | CEN ARS URA3 GFP | [24] |
pGK26 | CEN ARS URA3 SAC1 5' UTR (-500/-1)-GFP | [24] |
pAK29 | CEN ARS URA3 SAC1 5' UTR (-242/-1)-GFP | This study |
pAK30 | CEN ARS URA3 SAC1 5' UTR (-170/-1)-GFP | This study |
pAK34 | CEN ARS URA3 SAC1 5' UTR (-500/-150)-GFP | This study |
pAK36 | CEN ARS URA3 SAC1 5' UTR (-125/-1)-GFP | This study |
pAK38 | CEN ARS URA3 SAC1 5' UTR (-83/-1)-GFP | This study |
PAK40 | CEN ARS URA3 SAC1 5' UTR (-114/-1)-GFP | This study |
pAK42 | CEN ARS URA3 SAC1 5' UTR (-100/-1)-GFP | This study |
pAK47 | CEN ARS URA3 SAC1 5' UTR Δ(-100/-84)-GFP | This study |
pAK48 | CEN ARS URA3 SAC1 5' UTR Δ(-83/-70)-GFP | This study |
pAK49 | CEN ARS URA3 SAC1 5' UTR Δ(-91/-84)-GFP | This study |
pAK50 | CEN ARS URA3 SAC1 5' UTR Δ(-100/-92)-GFP | This study |
Strain | ||
ATY201 | MATαtrp1-delta901 leu2-3,112 his3-delta200 ura3-52 lys2-801 suc2-delta9 can1::hisG | [36] |
STY39 | MATa trp1-delta901 leu2-3,112 his3-delta200 ura3-52 lys2-801 suc2-delta9 can1::hisG sac1::TRP | [8] |
STY40 | MATa leu2-3, 112 ura3-52 his3-delta200 trp1-delta901 lys2-801 suc2-delta9 sac1::TRP1 stt4::HIS3 pSTT4-4 (LEU2 CEN6 stt4-4) | [8] |
STY47 | Pik1::ADE2-1 sac1::TRP YEplac181::pik1-12 | [8] |
PMY434 | MATαtrp1-delta901 leu2-3,112 his3-delta200 ura3-52 lys2-801 suc2-delta9 can1::hisG opi1::HIS3 | This study |
PMY435 | MATa trp1-delta901 leu2-3,112 his3-delta200 ura3-52 lys2-801 suc2-delta9 can1::hisG sac1::TRP opi1::HIS3 | This study |