Figure 3
From: Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate
Identification of a 9-bp element critical for SAC1 promoter activity. (A) Diagram depicting deletion constructs. The constructs were fused to the open reading frame of GFP in a CEN-based vector. (B) Expression of the GFP reporter. The respective plasmids were introduced into a wild-type strain background and promoter activity was determined by measuring relative GFP expression levels. Cell extracts were analyzed by SDS-PAGE and immunoblotting using anti-GFP and anti-glucose-6-phosphate dehydrogenase (Zwf1p) antibodies.