Figure 6
From: Iron-dependent degradation of IRP2 requires its C-terminal region and IRP structural integrity
The C-terminal region of IRP2 encompassing the C60 or C160 amino acids is not sufficient for iron-dependent degradation of a luciferase indicator. (A) Schematic representation of the luciferase fusion constructs harboring the C60 or C168 peptides of IRP2 (grey) at their C-termini. (B and C) H1299 cells engineered to express these constructs (in two independent clones) were treated overnight (14 h) with the indicated concentrations of FAC and lysates were subjected to Western blotting with luciferase (top) and β-actin (bottom) antibodies.