Figure 2
From: Iron-dependent degradation of IRP2 requires its C-terminal region and IRP structural integrity
The C-terminal domain 4 of IRP2 sensitizes IRP1/IRP2 chimeras for iron-dependent degradation. (A) Schematic representation of wild type IRP1 (white) and IRP2 (grey), the IRP21–3-IRP14 and IRP11–3-IRP24 chimeras and the ΔD4 IRP1 deletion mutant; the IRP1 constructs are tagged with FLAG and the others with HA epitopes. (B-E) H1299 cells engineered to express wild type IRP1, ΔD4 IRP1, wild type IRP2 or the above chimeras (in three independent clones) were treated overnight (14 h) with 30 μg/ml FAC or 100 μM hemin, and lysates were subjected to Western blotting with FLAG or HA (top) and β-actin (bottom) antibodies.