Table 3 Percentage of clones with both exchanges obtained with different oligonucleotides

Oligonucleotide

Distance of position 147 to the 3'-end (number of nucleotides)

number of clones per 10 ⁶ transfected cells

% of clones with both exchanges

C47-153-147

6

22

16

C53-153-147

12

13,3

54

C59-153-147

18

9,7

63

C65-153-147

24

8,7

47

C71-153-147

30

6,3

62

Positions 151 + x (TA-clamps)

Oligonucleotide

Distance (nucleotides) to the first position

number of clones per 10 ⁶ transfected cells

% of clones with both exchanges

H1-151

-

4.86

-

H7-151-141

-10

4.00

33

H5-151-144

-7

1.67

50

H3-151-147

-4

1.83

91

H2-151-150

-1

1.33

100

H4-151-156

5

2.67

63

H6-151-159

8

1.83

27

H8-151-165

14

4.00

8

H9-151-168

17

6.00

0

Positions 153 + x (TA-clamps)

Oligonucleotide

Distance (nucleotides) to the first position

number of clones per 10 ⁶ transfected cells

% of clones with both exchanges

H153-1T

-

18.00

-

C-153-147

-6

4.67

50

C-153-150

-3

1.00

100

C-153-156

3

2.67

89

C-153-159

6

5.00

35

Positions 151 + x (PTO modifications)

Oligonucleotide

Distance (nucleotides) to the first position

number of clones per 10 ⁶ transfected cells

% of clones with both exchanges

O45-151-147

-4

3.50

64

O45-151-150

-1

0.33

100

O42-151-156

5

6.50

41

O45-151-159

8

1.33

8

Positions 151 + 159, different lengths (PTO modifications)

Oligonucleotide

Length of the oligonucleotides (nucleotides)

number of clones per 10 ⁶ transfected cells

% of clones with both exchanges

O25-151-159

25

2.83

12

O30-151-159

30

3.83

13

O45-151-159

45

1.33

13

O48-151-159

48

2.00

17