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Table 3 Percentage of clones with both exchanges obtained with different oligonucleotides

From: Simultaneous targeted exchange of two nucleotides by single-stranded oligonucleotides clusters within a region of about fourteen nucleotides

Positions 153 and 147 (in different distances to the 3'-end)
Oligonucleotide Distance of position 147 to the 3'-end (number of nucleotides) number of clones per 10 6 transfected cells % of clones with both exchanges
C47-153-147 6 22 16
C53-153-147 12 13,3 54
C59-153-147 18 9,7 63
C65-153-147 24 8,7 47
C71-153-147 30 6,3 62
Positions 151 + x (TA-clamps)
Oligonucleotide Distance (nucleotides) to the first position number of clones per 10 6 transfected cells % of clones with both exchanges
H1-151 - 4.86 -
H7-151-141 -10 4.00 33
H5-151-144 -7 1.67 50
H3-151-147 -4 1.83 91
H2-151-150 -1 1.33 100
H4-151-156 5 2.67 63
H6-151-159 8 1.83 27
H8-151-165 14 4.00 8
H9-151-168 17 6.00 0
Positions 153 + x (TA-clamps)
Oligonucleotide Distance (nucleotides) to the first position number of clones per 10 6 transfected cells % of clones with both exchanges
H153-1T - 18.00 -
C-153-147 -6 4.67 50
C-153-150 -3 1.00 100
C-153-156 3 2.67 89
C-153-159 6 5.00 35
Positions 151 + x (PTO modifications)
Oligonucleotide Distance (nucleotides) to the first position number of clones per 10 6 transfected cells % of clones with both exchanges
O45-151-147 -4 3.50 64
O45-151-150 -1 0.33 100
O42-151-156 5 6.50 41
O45-151-159 8 1.33 8
Positions 151 + 159, different lengths (PTO modifications)
Oligonucleotide Length of the oligonucleotides (nucleotides) number of clones per 10 6 transfected cells % of clones with both exchanges
O25-151-159 25 2.83 12
O30-151-159 30 3.83 13
O45-151-159 45 1.33 13
O48-151-159 48 2.00 17
  1. The number of HAT resistant clones per 106 transfected cells and the percentage of clones showing the simultaneous exchange of both mismatches is given depending on the distance of the second mismatch nucleotide to the 3'-end of the oligonucleotide, the distance of the second mismatch to the first position or length of the oligonucleotides.