Sequences of coding strand of hprt intron 2 and exon 3 in V79 wildtype and V79-151 cells. | ||
---|---|---|
V79 wildtype | position 151 | |
DNA sequence | 5'-ttgtag G ACT GAA AGA CTT GCC C GA GAT GTC ATG AAA GAG ATG GGA-3 | |
amino acids | Thr Glu Arg Leu Ala Arg Asp Val Met Lys Glu Met Gly | |
V79-151 | position 151 | |
DNA sequence | 5'-ttgtag G ACT GAA AGA CTT GCC T GA GAT GTC ATG AAA GAG ATG GGA-3 | |
amino acids | Thr Glu Arg Leu Ala Stop Asp Val Met Lys Glu Met Gly | |
Positions of the intended first nucleotide exchanges for correcting the hprt mutation. | ||
 | Exchange of nucleotide on position 151 | |
DNA sequence | 5'-ttgtag G ACT GAA AGC CTT GCC C GA GAT GTT ATG AAA GAG ATG GGA-3' | |
amino acids | Thr Glu Arg Leu Ala Arg Asp Val Met Lys Glu Met Gly | |
 | Exchange of nucleotide on position 153 | |
DNA sequence | 5'-ttgtag G ACT GAA AGC CTT GCC TGC GAT GTT ATG AAA GAG ATG GGA-3' | |
amino acids | Thr Glu Arg Leu Ala Cys Asp Val Met Lys Glu Met Gly | |
Positions of the intended second nucleotide exchange generating a silent mutation | ||
Position | Base exchange | Sequence of coding strand of hprt intron 2 in V79-151 cells. |
141 | A to G | 5'-ttgtag G ACT GAG AGA CTT GCC T GA GAT GTC ATG AAA GAG ATG GGA-3 |
144 | A to G | 5'-ttgtag G ACT GAA AGG CTT GCC T GA GAT GTC ATG AAA GAG ATG GGA-3 |
147 | T to C | 5'-ttgtag G ACT GAA AGA CTC GCC T GA GAT GTC ATG AAA GAG ATG GGA-3 |
150 | C to T | 5'-ttgtag G ACT GAA AGA CTT GCT T GA GAT GTC ATG AAA GAG ATG GGA-3 |
156 | T to C | 5'-ttgtag G ACT GAA AGA CTT GCC T GA GAC GTC ATG AAA GAG ATG GGA-3 |
159 | C to T | 5'-ttgtag G ACT GAA AGA CTT GCC T GA GAT GTT ATG AAA GAG ATG GGA-3 |
165 | A to G | 5'-ttgtag G ACT GAA AGA CTT GCC T GA GAT GTC ATG AAG GAG ATG GGA-3 |
168 | G to A | 5'-ttgtag G ACT GAA AGA CTT GCC T GA GAT GTC ATG AAA GAA ATG GGA-3 |