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Figure 3 | BMC Molecular Biology

Figure 3

From: Simultaneous targeted exchange of two nucleotides by single-stranded oligonucleotides clusters within a region of about fourteen nucleotides

Figure 3

Correction rate of the first mismatch position. Correction rate of the first mismatch position after transfection with TA-clamp modified oligonucleotides carrying one mismatch (striped column) or two mismatches (black columns). The first exchange nucleotide was at hprt position 151 and the second mismatch at various distances. The X-axis indicates the distance of the second mismatch from the first exchange position in upstream and downstream direction. The Y-axis shows the mean number of clones obtained per 106 transfected cells. Mean and standard deviations were calculated based on the total number of clones after 3 – 6 transfection experiments per oligonucleotide (Additional file 1).

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