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Table 2 Hypertonicity threshold required to activate the endogenous 9xNFAT-Luc reporter in transgenic lymphocytes.

From: Analysis of the transcriptional activity of endogenous NFAT5 in primary cells using transgenic NFAT-luciferase reporter mice

   9xNFAT-Luc reporter activity (RLU)
Preactivation with ConA + IL2 Stimulus Experiment # 1 Experiment # 2 Experiment # 3 Experiment # 4
24 hours 300 mOsm/kg (isotonic) 398 446 761 662
  340 mOsm/kg 295 673 1628 690
  360 mOsm/kg 1254 933 1648 944
  380 mOsm/kg 11468 1622 6060 2353
  400 mOsm/kg 38768 22808 11061 6494
  PMA + ionomycin (isotonic) 7039 20428 12081 6647
48 hours 300 mOsm/kg (isotonic) 257 351 329 210
  340 mOsm/kg 159 397 441 311
  360 mOsm/kg 205 327 567 226
  380 mOsm/kg 1118 1121 1204 1258
  400 mOsm/kg 7105 7997 1891 3291
  PMA + ionomycin (isotonic) 2079 19702 1803 3638
72 hours 300 mOsm/kg (isotonic) 155 356 113 146
  340 mOsm/kg 136 357 131 214
  360 mOsm/kg 128 342 196 99
  380 mOsm/kg 188 379 260 148
  400 mOsm/kg 288 467 458 288
  PMA + ionomycin (isotonic) 11359 22321 2389 1511
  1. Transgenic 9xNFAT-Luc splenocytes were activated with concanavalin A plus IL2 during 24 to 72 hours in isotonic medium, and then exposed to hypertonicity, or stimulated with PMA plus ionomycin for 24 hours. Luciferase activity is represented as relative light units per second (RLU) after normalization with endogenous lactate dehydrogenase.