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Table 1 Impaired activation of endogenous 9xNFAT-Luc transgenic reporter by hypertonicity in NFAT5-deficient lymphocytes.

From: Analysis of the transcriptional activity of endogenous NFAT5 in primary cells using transgenic NFAT-luciferase reporter mice

   9xNFAT-Luc reporter activity (RLU)
   Experiment # 1 Experiment # 2 Experiment # 2
   NFAT5 +/+ NFAT5 -/- NFAT5 +/+ NFAT5 -/- NFAT5 +/+ NFAT5 -/-
8-hour stimulation       
300 mOsm/kg (isotonic) - FK506 19 25 45 19 16 16
  + FK506 11 13 18 16 7 8
430 mOsm/kg (hypertonic) - FK506 1026 117 825 191 387 27
  + FK506 720 83 1316 13 579 24
480 mOsm/kg (hypertonic) - FK506 12616 540 5677 42 10901 273
  + FK506 19543 435 2894 51 7403 102
PMA+ ionomycin (isotonic) - FK506 2445 10087 2080 2494 1726 6364
  + FK506 24 19 34 31 37 158
24-hour stimulation       
300 mOsm/kg (isotonic) - FK506 21 21 21 20 19 33
  + FK506 10 15 14 15 12 10
430 mOsm/kg (hypertonic) - FK506 1351 188 297 239 348 102
  + FK506 550 122 254 129 388 73
480 mOsm/kg (hypertonic) - FK506 6930 359 1742 224 7267 245
  + FK506 1507 232 1111 129 3977 129
PMA+ ionomycin (isotonic) - FK506 1100 2516 791 825 418 1775
  + FK506 15 17 16 8 30 30
  1. Luciferase activity was measured in proliferating transgenic 9xNFAT-Luc T cells derived from littermate NFAT5+/+ and NFAT5-/- mice after stimulation with hypertonic medium or PMA plus ionomycin, in the absence or presence of 100 nM FK506. Luciferase activity is represented as relative light units per second (RLU) after normalization with endogenous lactate dehydrogenase.