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Figure 4 | BMC Molecular Biology

Figure 4

From: Analysis of the transcriptional activity of endogenous NFAT5 in primary cells using transgenic NFAT-luciferase reporter mice

Figure 4

Hypertonicity threshold required to activate the 9xNFAT-Luc reporter in different cell types. A) Luciferase activity in 9xNFAT-Luc transgenic splenocytes treated during 24 hours with hypertonicity or PMA plus ionomycin, after having been preactivated with concanavalin A plus IL2 during 24 hours, 48 hours or 72 hours. One representative experiment is shown out of three performed independently (see Table 2). B) Luciferase activity in 9xNFAT-Luc transgenic bone marrow-derived macrophages from NFAT5+/+ and NFAT5-/- mice exposed to increasing hypertonicity levels during 24 hours. Mean ± S.D of two independent experiments is shown. C) Luciferase activity in 9xNFAT-Luc transgenic bone marrow-derived macrophages exposed to increasing hypertonicity levels during 24 hours, in the absence or presence of FK506 (100 nM). Mean ± S.D of three independent experiments is shown. D) Luciferase activity in 9xNFAT-Luc transgenic mouse embryo fibroblasts exposed to increasing hypertonicity levels during 24 hours. Mean ± S.D of three independent experiments is shown. N.S.: non statistically significant.

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