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Figure 2 | BMC Molecular Biology

Figure 2

From: Analysis of the transcriptional activity of endogenous NFAT5 in primary cells using transgenic NFAT-luciferase reporter mice

Figure 2

Unresponsiveness of the 9xNFAT-Luc reporter to hypertonicity in NFAT5-deficient cells. A) NFAT5+/+ and NFAT5-/- MEF were transfected with the 9xNFAT-Luc reporter and stimulated with PMA plus ionomycin or hypertonicity (460 mOsm/kg), with or without FK506, during 24 hours. Mean ± S.D of three independent experiments is shown. B) NFAT5-/- MEF were transfected with the 9xNFAT-Luc reporter and an empty vector (pBSK(+)) or an NFAT5 expression vector. Cells were stimulated with PMA plus ionomycin or hypertonicity (460 mOsm/kg), with or without FK506, during 24 hours. Mean ± S.E.M of three independent experiments is shown.

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BMC Molecular Biology

ISSN: 1471-2199

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