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Figure 3 | BMC Molecular Biology

Figure 3

From: Differential regulation of the two transcriptional activation domains of the coiled-coil coactivator CoCoA by sumoylation

Figure 3

Identification of SUMO1 conjugation site in CoCoA. (A) COS-7 cells were co-transfected with plasmids expressing EGFP-SUMO1 (200 ng), PIAS1 (100 ng), and full-length HA-CoCoA wild type or mutants (800 ng) as indicated. (B) 293T cells were transfected with plasmids expressing EGFP-SUMO1 (200 ng), PIAS1 (100 ng), and HA-CoCoA(1–190) wild type or K29R mutant (800 ng) as indicated. Cell extracts were collected 48 h after transfection and analyzed by immunoblot with anti-HA antibody. (C) COS-7 cells were co-transfected with plasmids expressing Myc-SUMO2 (200 ng), PIAS1 (100 ng), and full-length HA-CoCoA wild type or mutants (800 ng) as indicated. Cell extracts were collected 48 h after transfection and analyzed by immunoblot with anti-HA antibody.

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