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Figure 2 | BMC Molecular Biology

Figure 2

From: Differential regulation of the two transcriptional activation domains of the coiled-coil coactivator CoCoA by sumoylation

Figure 2

PIAS1 and ARIP3 act as SUMO E3 ligases for CoCoA. COS-7 cells were co-transfected with plasmids expressing HA-CoCoA or HA-CoCoA(1–190) (800 ng), EGFP-SUMO1 (200 ng), and PIAS1 or ARIP3 (100 ng) as indicated. Cell extracts were collected 48 h post-transfection and analyzed by immunoblot with anti-HA antibody. Arrowheads indicate the SUMO-conjugated CoCoA or CoCoA(1–190).

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