BRD7 minimal promoter selectively functions in some cell lines with weak expression of c-Myc. (A) Luciferase assay of pGL3/-266,-212 in different cultured cell lines. Luciferase activity of pGL3/-404,+46 and pGL3/-266,-212 is represented by black and gray histograms, respectively. (B) Bioinformatics analysis of putative cis-acting elements in BRD7 minimal promoter region -266/-212 by MatInspector program. "+1" represents the A of the initiation translation codon. (C) Detection of c-Myc, E2F6 expression in nine analyzed cell lines by RT-PCR. (D) Detection of Sp1, c-Myc and E2F6 expression in nine analyzed cell lines by western blot assay.