Identificating of BRD7 minimal promoter (-266/-212). (A) Luciferase assay of deletion constructs of BRD7 promoter. Luciferase activity in COS7 and 5-8F cells is represented by black and gray histograms, respectively. (B) Detection of GFP fluorescence of modified construct pGL3/-266,-212/EGFP in COS7 and 5-8F cells by direct GFP fluorescence assay. Full-length modified promoter construct pGL3/-404,+46/EGFP was used as a positive control. A1, B1: COS7 cells transfected with pGL3/-404,+46/GFP; A2, B2: COS7 cells transfected with pGL3/-266,-212/GFP; A3, B3: 5-8F cells transfected with pGL3/-404,+46/GFP; A4, B4: 5-8F cells transfected with pGL3/-266,-212/GFP.