Table 1 Sequence of synthetic oligonucleotides used in this study

P1/USFmer

5'-GGCGGCCCACGTG TCCACCT-3'

USFmer ^b

5'-GGCCAGACCACGTG GTCTGTTC-3'

Sp1mer ^b

5'-CCCTTGGTGGGGGCGGGGCCTAAGCTGCG-3'

MyoDmer ^c

5'-CCCCCCAACACCTGCTGCCTGA-3'

P1/USFmut

5'-CCCACGGGCGGCCACA GTGTCCACCTATTC-3'

P1/Spmut

5'-GTGAGCATA GT CGTA GAGCAT GGT AGA GTACCGC-3'

Q-PCR Primers

Amplified region

Sequences

P1 ChIP f

P1 promoter AβH-J-J

5'-AACGAACTAAATTCCTGCTTCAGC-3'

P1 ChIP r

(contains USF elements)

5'-TCCTTTGGAACGTGGCAAACGT-3'

Neg ChIP f

negative control region

5'-TGTGTGATTTCCCGTCAACTGTC-3'

Neg ChIP r

(without USF elements)

5'-CCAGCCTCTTCCATTGGATACAA-3'

E1 f

from exon 1 to 5

5'-CAAGAGCAGCGGCAACAG-3'

E 5r

of AβH-J-J transcripts

5'-AATAAAACTTTGGCATCATCCACTCAAAATCTCC-3'

qUSF1 f

USF1 transcripts

5'-TGATGATGCAGTTGACACGGA-3'

qUSF1 r

5'-CAGTTGTTGATCTTGTCTCGGC-3'

GAPDH f

glyceraldehyde-3-phosphate

5'-AAGGTCGGAGTCAACGGATTT-3'

GAPDH r

dehydrogenase mRNA

5'-ACTGTGGTCATGAGTCCTTCCA-3'

HMBS f

hydroxymethyl-bilane

5'-GAACATGCCCTGGAGAAGAATGA-3'

HMBS r

synthase mRNA

5'-GGTAGCCTGCATGGTCTCTTGTAT-3'

ACTIN f

actin mRNA

5'-TGACGGGGTCAACCACACTGTGCCCATCTA-3'

ACTIN r

5'-CTAGAAGCATTTGCGGTGGACGATGGAGGG-3'