Figure 8

Effect of USF1 silencing obtained by short interference RNA on P1 promoter activity. HeLa cells were transiently transfected with USF1 shRNA vector, with Scramble shRNA vector or vector in which no small hairpin DNA oligo was cloned (Null shRNA vector). Two days after transient transfection of these vectors into HeLa cells, total RNA was extracted and used for quantitative Real Time RT-PCR, using primers targeting USF1 mRNA (panel A) or P1 promoter specific transcripts (panel B). Results represent the average ± SD of three independent experiments carried out in duplicate; ΔΔCt method was used to compare gene expression data, standard error of the mean was calculated; statistical significance: *P < 0.05.