Figure 3

AβH-J-J P1 promoter activity in HeLa cell line. HeLa cells were transiently transfected with sequentially deleted reporter constructs of the -661/+81 P1 nucleotide sequence (represented in the left side of the figure, F-M). Transient transfection and luciferase assays were performed in triplicate, and the data (right side of the figure) were normalized to renilla luciferase activity and are shown as relative activities compared to that for pGL3-basic, a reporter vector with a basal promoter. The values are the means ± SD of at least three independent experiments.