Figure 2

Quantitative Real-time RT-PCR analysis of the transcripts starting from P1 promoter in human cell lines. (A) The exon 1 starting mRNAs of AAH and humbug, the PCR primers and the 297 bp PCR product are represented; white boxes indicate exons common to the two transcripts. (B) Relative mRNA expression levels of P1 promoter transcripts were evaluated by Quantitative real-time RT-PCR in which cDNA derived from human cell lines (a, MCF7; b, Colo 38; c, Hek293; d, HepG2; e, HeLa; f, RD; g, MDA) total RNA were amplified. Relative quantification is expressed as normalized fold expression and was calculated by using MCF7 as reference sample (value = 1). All data represent the mean of three determinations from two independent experiments; *P < 0.001.