Figure 8
From: Ror2 modulates the canonical Wnt signaling in lung epithelial cells through cooperation with Fzd2
The CRD and not the PRD domain of Ror2 is required for Ror2-Fzd2 cooperation. H441 cells were transfected with pSV-β-gal (Promega, WI), STF, Fzd2 and expression constructs for Ror2 wild-type and mutant derivatives, and then treated with L-CM or Wnt3aCM. Equal amounts of DNA and conditioned media were used in each assay. Luciferase values were normalized to beta-galactosidase values for transfection efficiency. Data shown are the average ratios of normalized luciferase values in Wnt3aCM-treated cells over that of L-CM-treated cells. * indicates p < 0.05 when compared with data of bar 2. *** indicates p < 0.05 when compared with data of bar 3. ‡ indicates p < 0.05 when compared with data of bar 1. ‡‡‡ indicates p < 0.05 when compared with data of bar 8.