Figure 5

Ror2 cooperates with Fzd2 but not Fzd7 in mediating Wnt3a-stimulated canonical Wnt signaling in H441 cells. H441 cells were transfected with pSV-β-gal (Promega, WI), STF, plus either Fzd2 or Fzd7 expression constructs with or without Ror2WT plasmid and then treated with conditioned media, L-CM or Wnt3aCM. Equal amounts of DNA and conditioned media were used in each assay. Luciferase values were normalized to beta-galactosidase values for transfection efficiency. Values of the assays treated with L-CM alone (empty bars) were adjusted to unity to normalize all other experimental values in the same group. * indicates p < 0.05.