Figure 4

Ror2 potentiates Wnt3a stimulation of canonical Wnt signaling. H441 cells were transfected with pSV-β-gal (Promega, WI) and STF, plus expression constructs for Ror2 wild-type (Ror2WT). An equal quantity of control vector was used as negative control. Twenty-four hours after transfection, cells were treated with L-CM and Wnt3aCM for 2 hrs, 4 hrs, 6 hrs or 16 hrs. Luciferase values representing STF activities were normalized to beta-galactosidase for transfection efficiency. Data shown are the average ratios of normalized luciferase values in Wnt3aCM-treated cells over that of L-CM-treated cells. * indicates p < 0.05.