Figure 3

Fzd2 and Fzd7, but not Fzd6 mediate Wnt3a stimulation of canonical Wnt signaling. H441 cells were transfected with pSV-β-gal (Promega, WI) and STF, plus expression constructs for Fzd2, Fzd6, or Fzd7. An equal quantity of control vector was used as negative control. Twenty-four hours after transfection, cells were treated with L-CM and Wnt3aCM. Two dilutions of Wnt3aCM (1/8 or 1/3) were applied. Equal amounts of conditioned media were used in each assay. Luciferase values representing STF activities were normalized to beta-galactosidase for transfection efficiency. Values of the assays treated with L-CM alone were adjusted to unity to normalize all other experimental values in the same group. * indicates p < 0.05 when compared with data of bar 2. ‡ indicates p < 0.05 when compared with data of bar 3.