Figure 12
From: Ror2 modulates the canonical Wnt signaling in lung epithelial cells through cooperation with Fzd2
Wnt3a stimulation of STF mediated by Fzd2 and Ror2 is inhibited by GSK3. H441 cells were transfected with pSV-β-gal (Promega, WI), STF, Fzd2 and Ror2WT plus Xgsk3 expression construct, and then treated with L-CM or Wnt3aCM. Equal amounts of DNA and conditioned media were used in each assay. Luciferase values were normalized to beta-galactosidase for transfection efficiency. Values of the assay transfected with control vector and treated with L-CM alone (empty bars) were adjusted to unity to normalize all other experimental values in the same group. * indicates p < 0.05.