Figure 11
From: Ror2 modulates the canonical Wnt signaling in lung epithelial cells through cooperation with Fzd2
Wnt3a stimulation of STF mediated by Fzd2 and Ror2 is inhibited by Dkk1 and Krm1. H441 cells were transfected with pSV-β-gal (Promega, WI), STF, Fzd2 and Ror2WT with and without Dkk1 and Krm1 expression constructs, and then treated with conditioned media, L-CM or Wnt3aCM. Equal amounts of DNA and conditioned media were used in each assay. Luciferase values were normalized to beta-galactosidase for transfection efficiency. Values of the assay transfected with control vector and treated with L-CM alone (empty bars) were adjusted to unity to normalize all other experimental values in the same group. * indicates p < 0.05.