Figure 10
From: Ror2 modulates the canonical Wnt signaling in lung epithelial cells through cooperation with Fzd2
Wnt3a stimulation of STF mediated by Fzd2 and Ror2 is inhibited by Lrp6 siRNA. A. H441 cells were transfected with pSV-β-gal (Promega, WI), STF, Fzd2 and Ror2WT with either Lrp6 siRNAs or negative control siRNAs, and then treated with conditioned media, L-CM or Wnt3aCM. Equal amounts of DNA, siRNAs and conditioned media were used in each assay. Luciferase values were normalized to beta-galactosidase for transfection efficiency. Data shown are the average ratios of normalized luciferase values in Wnt3aCM-treated cells over that of L-CM-treated cells. * indicates p < 0.05. B. Western blot analysis of levels of Lrp6, Lrp5 and alpha-tubulin in H441 cells transfected with either Lrp6 siRNA or negative control siRNA (Ctrl).