Wnt3a stimulation of STF mediated by Fzd2 and Ror2 is inhibited by Lrp6 siRNA. A. H441 cells were transfected with pSV-β-gal (Promega, WI), STF, Fzd2 and Ror2WT with either Lrp6 siRNAs or negative control siRNAs, and then treated with conditioned media, L-CM or Wnt3aCM. Equal amounts of DNA, siRNAs and conditioned media were used in each assay. Luciferase values were normalized to beta-galactosidase for transfection efficiency. Data shown are the average ratios of normalized luciferase values in Wnt3aCM-treated cells over that of L-CM-treated cells. * indicates p < 0.05. B. Western blot analysis of levels of Lrp6, Lrp5 and alpha-tubulin in H441 cells transfected with either Lrp6 siRNA or negative control siRNA (Ctrl).