Figure 7

In vitro binding of Tc UBP1 and Tc RBP3 to a common target. A) HYPO1 was identified in both libraries. The interaction with each protein was confirmed by RT-PCR assays in IP samples. B) Scheme of HYPO1 mRNA, 3' UTR sequence and location of UBP1m (black or bold) and RBP3m (gray) are shown. In vitro transcribed region is marked with arrows. The numbers indicate distances from stop codon where motifs were found. C) In vitro binding reactions were performed incubating the biotinylated transcript with both recombinant proteins in the same binding reaction at the concentrations indicated.