Figure 3

Validation of experimental transcripts. A) IP assays and semiquantitative RT-PCR amplification (25–30 cycles) were done to test library and non-related targets (RBP3) in the fraction of mRNAs bound to each protein. Specific set of primers shown at the right side of each panel were used. Only representative dilutions are shown. B) Abundance of transcripts present in Tc UBP1 and Tc RBP3 IP samples was assessed by real-time RT-PCR. Two mRNA targets for each protein in each IP sample were quantified. The relations of Tc UBP1 over Tc RBP3 targets in Tc UBP1, and in Tc RBP3 IP samples are plotted. The inverse relations are also shown. C) Parasites were in vivo treated with formaldehyde to maintain endogenous mRNP previous to IP and RT-PCR assays. PS, preimmune serum.