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Table 3 Oligonucleotides used in the study

From: Factors acting on Mos1 transposition efficiency

Primer Name Nucleic acid sequence
End modification of the kanamycin resistance gene
KanaSal1 5'-AGCGTCGCATACAAGGGGTGTTATGAGCCAT-3'
KanaHind3 5'-ACCCAAGCTTAATCGGGGGCTCCCTTTAGG-3'
3Kana3 junction from the kanamycin resistance gene
KanaJunc1 5'-TCATCTGTATAACATCATTGGC-3'
KanaJunc2 5'-CATGGTGATTTCTCACTTG-3'
Stuffer fragments amplified from DpAV4 sequence referenced as AJ279812
3primeXba1 5'-GCTCTAGACGGACGTCCAATACATGATG-3'
5primeXba1-F1 5'-GCTCTAGACCGAAAAGATAGACAGTGTG-3'
5primeXba1-F2 5'-GCTCTAGAAACGTGACGCACATGGCTAT-3'
5primeXba1-F3 5'-GCTCTAGAAAACATCCTTCGCCCTGAAC-3'
5primeXba1-F4 5'-GCTCTAGAAGGATTGGTGGGATTTTCCG-3'
5primeXba1-F5 5'-GCTCTAGAACGGCAACTTTCGGAACTAC-3'
Test-orient-F 5'-GCGAATTGGCCCCTAGATTT-3'
  Insertion site analyses in the ccdB gene
ccdBup 5'-AGTCGTTCGGCTTCATCTGG-3'
ccdBdown 5'-ATCAGGAAGGGATGGCTGAG-3'
  ITR cloning for circular permutation
5'ITRup 5'-AGCTCGTTTACCAGGTGTACAAGTAGGGAATGTCGGTTCCC-3'
5'ITRdw 5'-GGGAACCGACATTCCCTACTTGTACACCTGGTAAACGAGCT-3'
3'ITRup 5'-AGCTCGTTTATCAGGTGTACAAGTATGAAATGTCGTTTCCC-3'
3'ITRdw 5'-GGGAAACGACATTTCATACTTGTACACCTGATAAACGAGCT-3'
  Fragments for circular permutation
O1 5'-GGGTTTTCCCAGTCACG-3'
O1R 5'-AACGACATTTCATACTTGTA-3'
O2 5'-ACGTTGTAAAACGACGGC-3'
O2R 5'-CTAGAGCGGCCGCGG-3'
O3 5'-CCAGTGAGCGCGCGTA-3'
O3R 5'-CGGGGGATCCACTAGTT-3'
O4 5'-TACGACTCACTATAGGGC-3'
O4R 5'-ATCGAATTCCTGCAGCCC-3'
O5 5'-CGAATTGGAGCTCGTTTAT-3
O5R 5'-GTATCGATAAGCTTGATATC-3'
O6 5'-ATCAGGTGTACAAGTATGAA-3'
O6R 5'-TTCCCTCGAGGTCGACG-3'