Figure 3
Cleavage positions on preformed PEC1 and SEC2. (a) The DNA contained in SEC2 and PEC1 was eluted from EMSA gel and analyzed on a sequencing gel. An asterisk marks the position of the 32P. The sequence reaction (G+A) of each probe was loaded as a marker, and the sequence was indicated in the left margin. Brackets indicate the location of the ITR region, the flanking TA dinucleotide is shown in bold, upper-case letters, and the non-ITR flanking DNA is shown in lower-case letters. (Left panel) Cleavage products obtained using ITR70α, labeled on the non-transferred strand as a probe. (Right panel) Cleavage products obtained using ITR70γ, labeled on the transferred strand as a probe. (b) Sequence of the DNA used in cleavage-site determination. The "minor" cleavage sites at the outer ITR extremity are indicated by black diamonds, and the major site by black circle, located either on the non-transferred strand (NT) or on the transferred strand (T). Cleavage located at the inner ITR extremity of the T strand is indicated by a gray diamond.