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Figure 2 | BMC Molecular Biology

Figure 2

From: The artiodactyl APOBEC3 innate immune repertoire shows evidence for a multi-functional domain organization that existed in the ancestor of placental mammals

Figure 2

The A3 genomic repertoire of sheep, cattle and pigs. (A) An illustration of the A3 genes of the indicated mammals. Z1, Z2 and Z3 domains are colored green, orange and blue, respectively. The conserved flanking genes CBX6 and CBX7 are shown and the scale is indicated. Solid lines represent finished sequence and dotted lines represent gaps or incomplete regions. Non-mammalian vertebrates such as frogs lack A3 genes. (B) A dotplot analysis shows A3Z1 in cattle but not in pig genomic sequence. The x- and y-axis numbers designate nucleotide positions within the indicated genomic consensus sequences. (C) PCR analysis of genomic DNA from the indicated species showing that a 250–256 bp Z1-specific amplicon can only be obtained from a subset of mammals. The human 51 and 54 cycle amplicons were too abundant to be run on the same gel. Monkey genomic DNA is from the African green monkey. The ALDOA gene was used as a positive control (115 bp).

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BMC Molecular Biology

ISSN: 1471-2199

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