Table 3 Candidate reference genes for normalization of qRT-PCR (in lung cultured cells) ranked according to their expression stability by GeNorm and NormFinder programs
GeNorm | NormFinder | |||
|---|---|---|---|---|
Genes | Stability value | Genes | Stability value | |
PPIA | 0.377 | Most stable genes | PPIA | 0.078 |
RPLPO | 0.377 | RPLP0 | 0.199 | |
GAPDH | 0.387 | HPRT1 | 0.232 | |
18S | 0.430 | GAPDH | 0.244 | |
HPRT1 | 0.473 | 18S | 0.257 | |
GUSB | 0.509 | GUSB | 0.328 | |
POLR2A | 0.547 | POLR2A | 0.407 | |
HMBS | 0.580 | UBC | 0.408 | |
PGK1 | 0.614 | PGK1 | 0.420 | |
UBC | 0.644 | HMBS | 0.425 | |
ACTB | 0.681 | ACTB | 0.508 | |
B2M | 0.719 | B2M | 0.529 | |
TFRC | 0.761 | TFRC | 0.583 | |
YWHAZ | 0.801 | YWHAZ | 0.631 | |
IPO8 | 0.852 | IPO8 | 0.758 | |
TBP | 0.917 | Least stable genes | TBP | 0.863 |