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Table 3 Housekeeping gene expression following vehicle/hormone/toxicant treatment from 24–96 hpf, as measured by QPCR

From: Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment

Ct values +/- S.E.M.

GENE

CONTROL

ETOH

DMSO

0.1 μM E2

1 μM T

10 μM ICI

10 nM BNF

1 nM TCDD

bactin1

15.1 ± 0.07

14.9 ± 0.01

15.2 ± 0.12

15.0 ± 0.03

14.7 ± 0.08

15.1 ± 0.05

15.5 ± 0.19

15.1 ± 0.02

tuba1

19.9 ± 0.11

19.5 ± 0.08

19.6 ± 0.08

20.0 ± 0.13

19.3 ± 0.08

19.8 ± 0.04

19.7 ± 0.05

19.5 ± 0.08

gapdh

16.8 ± 0.12

16.1 ± 0.20*

16.0 ± 0.10*

15.9 ± 0.02

15.9 ± 0.07

15.8 ± 0.03

15.9 ± 0.11

16.1 ± 0.06

g6pd

21.4 ± 0.11

20.7 ± 0.04*

20.8 ± 0.03

21.5 ± 0.06

20.8 ± 0.01

20.8 ± 0.03

21.4 ± 0.16

21.1 ± 0.07

tbp

20.4 ± 0.53

21.6 ± 0.14

20.6 ± 0.31

21.4 ± 0.05

21.9 ± 0.15

21.9 ± 0.06

22.1 ± 0.13

22.3 ± 0.25

b2m

23.8 ± 0.16

23.0 ± 0.13

22.9 ± 0.23

23.6 ± 0.10

24.3 ± 0.43

24.0 ± 0.44

24.5 ± 0.30

24.2 ± 0.16

elfa

14.6 ± 0.12

14.2 ± 0.01*

14.2 ± 0.01*

14.2 ± 0.05

14.4 ± 0.12

14.3 ± 0.05

14.4 ± 0.07

14.3 ± 0.03

18s

16.2 ± 0.02

16.3 ± 0.08

16.8 ± 0.18*

16.3 ± 0.03

16.3 ± 0.03

16.3 ± 0.15

16.3 ± 0.02

16.9 ± 0.09

  1. Values represent Ct values (mean +/- SEM) from 3 biological replicates (50 embryos/larvae per pool, 3 pools per treatment group). For details, see Methods and Results. Asterisks (*) indicate significant difference between vehicle [dimethyl sulfoxide (DMSO), ethanol (EtOH)] and control (control = untreated; p < .05). Boldface indicates significant difference between treatment [17β-estradiol (E2), testosterone (T), ICI 182,780 (ICI), β-napthaflavone (BNF), tetrachlodibenzo-p-dioxin (TCDD)] and DMSO (p < .05).
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BMC Molecular Biology

ISSN: 1471-2199

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