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Table 3 Housekeeping gene expression following vehicle/hormone/toxicant treatment from 24–96 hpf, as measured by QPCR

From: Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment

Ct values +/- S.E.M.
GENE CONTROL ETOH DMSO 0.1 μM E2 1 μM T 10 μM ICI 10 nM BNF 1 nM TCDD
bactin1 15.1 ± 0.07 14.9 ± 0.01 15.2 ± 0.12 15.0 ± 0.03 14.7 ± 0.08 15.1 ± 0.05 15.5 ± 0.19 15.1 ± 0.02
tuba1 19.9 ± 0.11 19.5 ± 0.08 19.6 ± 0.08 20.0 ± 0.13 19.3 ± 0.08 19.8 ± 0.04 19.7 ± 0.05 19.5 ± 0.08
gapdh 16.8 ± 0.12 16.1 ± 0.20* 16.0 ± 0.10* 15.9 ± 0.02 15.9 ± 0.07 15.8 ± 0.03 15.9 ± 0.11 16.1 ± 0.06
g6pd 21.4 ± 0.11 20.7 ± 0.04* 20.8 ± 0.03 21.5 ± 0.06 20.8 ± 0.01 20.8 ± 0.03 21.4 ± 0.16 21.1 ± 0.07
tbp 20.4 ± 0.53 21.6 ± 0.14 20.6 ± 0.31 21.4 ± 0.05 21.9 ± 0.15 21.9 ± 0.06 22.1 ± 0.13 22.3 ± 0.25
b2m 23.8 ± 0.16 23.0 ± 0.13 22.9 ± 0.23 23.6 ± 0.10 24.3 ± 0.43 24.0 ± 0.44 24.5 ± 0.30 24.2 ± 0.16
elfa 14.6 ± 0.12 14.2 ± 0.01* 14.2 ± 0.01* 14.2 ± 0.05 14.4 ± 0.12 14.3 ± 0.05 14.4 ± 0.07 14.3 ± 0.03
18s 16.2 ± 0.02 16.3 ± 0.08 16.8 ± 0.18* 16.3 ± 0.03 16.3 ± 0.03 16.3 ± 0.15 16.3 ± 0.02 16.9 ± 0.09
  1. Values represent Ct values (mean +/- SEM) from 3 biological replicates (50 embryos/larvae per pool, 3 pools per treatment group). For details, see Methods and Results. Asterisks (*) indicate significant difference between vehicle [dimethyl sulfoxide (DMSO), ethanol (EtOH)] and control (control = untreated; p < .05). Boldface indicates significant difference between treatment [17β-estradiol (E2), testosterone (T), ICI 182,780 (ICI), β-napthaflavone (BNF), tetrachlodibenzo-p-dioxin (TCDD)] and DMSO (p < .05).
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