|
Ct values +/- S.E.M.
|
|---|
|
GENE
|
CONTROL
|
ETOH
|
DMSO
|
0.1 μM E2
|
1 μM T
|
10 μM ICI
|
10 nM BNF
|
1 nM TCDD
|
|---|
|
bactin1
|
15.1 ± 0.07
|
14.9 ± 0.01
|
15.2 ± 0.12
|
15.0 ± 0.03
|
14.7 ± 0.08
|
15.1 ± 0.05
|
15.5 ± 0.19
|
15.1 ± 0.02
|
|
tuba1
|
19.9 ± 0.11
|
19.5 ± 0.08
|
19.6 ± 0.08
|
20.0 ± 0.13
|
19.3 ± 0.08
|
19.8 ± 0.04
|
19.7 ± 0.05
|
19.5 ± 0.08
|
|
gapdh
|
16.8 ± 0.12
|
16.1 ± 0.20*
|
16.0 ± 0.10*
|
15.9 ± 0.02
|
15.9 ± 0.07
|
15.8 ± 0.03
|
15.9 ± 0.11
|
16.1 ± 0.06
|
|
g6pd
|
21.4 ± 0.11
|
20.7 ± 0.04*
|
20.8 ± 0.03
|
21.5 ± 0.06
|
20.8 ± 0.01
|
20.8 ± 0.03
|
21.4 ± 0.16
|
21.1 ± 0.07
|
|
tbp
|
20.4 ± 0.53
|
21.6 ± 0.14
|
20.6 ± 0.31
|
21.4 ± 0.05
|
21.9 ± 0.15
|
21.9 ± 0.06
|
22.1 ± 0.13
|
22.3 ± 0.25
|
|
b2m
|
23.8 ± 0.16
|
23.0 ± 0.13
|
22.9 ± 0.23
|
23.6 ± 0.10
|
24.3 ± 0.43
|
24.0 ± 0.44
|
24.5 ± 0.30
|
24.2 ± 0.16
|
|
elfa
|
14.6 ± 0.12
|
14.2 ± 0.01*
|
14.2 ± 0.01*
|
14.2 ± 0.05
|
14.4 ± 0.12
|
14.3 ± 0.05
|
14.4 ± 0.07
|
14.3 ± 0.03
|
|
18s
|
16.2 ± 0.02
|
16.3 ± 0.08
|
16.8 ± 0.18*
|
16.3 ± 0.03
|
16.3 ± 0.03
|
16.3 ± 0.15
|
16.3 ± 0.02
|
16.9 ± 0.09
|
- Values represent Ct values (mean +/- SEM) from 3 biological replicates (50 embryos/larvae per pool, 3 pools per treatment group). For details, see Methods and Results. Asterisks (*) indicate significant difference between vehicle [dimethyl sulfoxide (DMSO), ethanol (EtOH)] and control (control = untreated; p < .05). Boldface indicates significant difference between treatment [17β-estradiol (E2), testosterone (T), ICI 182,780 (ICI), β-napthaflavone (BNF), tetrachlodibenzo-p-dioxin (TCDD)] and DMSO (p < .05).
