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Table 2 Housekeeping gene expression in different tissues of adult male (M) and female (F) zebrafish, as measured by QPCR

From: Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment

Ct Values +/- S.E.M.

 

EYE

BRAIN

HEART

LIVER

MUSCLE

GONAD

GENE

M

F

M

F

M

F

M

F

M

F

M

F

bactin1

16.2 ± 0.45

16.4 ± 0.26

16.4 ± 0.33

16.4 ± 0.46

17.5 ± 0.33

16.7 ± 0.57

18.2 ± 0.42

17.7 ± 0.30

17.7 ± 0.58

16.8 ± 0.51

16.2 ± 0.29

14.2 ± 0.24

tuba1

23.2 ± 0.19

23.1 ± 0.17

22.2 ± 0.23

22.3 ± 0.18

22.3 ± 0.29

23.5 ± 0.43

27.8 ± 0.28

29.2 ± 0.27

27.0 ± 0.40

25.4 ± 0.18

21.2 ± 0.30

21.4 ± 0.85

gapdh

18.4 ± 0.33

17.8 ± 0.58

22.6 ± 0.98

23.6 ± 0.84

15.0 ± 0.35

15.2 ± 0.53

15.8 ± 0.62

16.8 ± 0.62

15.4 ± 0.07

14.8 ± 0.34

20.1 ± 0.78

15.0 ± 0.45

g6pd

21.2 ± 0.08

21.6 ± 0.17

21.8 ± 0.11

22.0 ± 0.38

22.2 ± 0.07

21.4 ± 0.19

20.3 ± 0.20

21.0 ± 0.12

25.1 ± 0.37

23.3 ± 0.11

20.4 ± 0.10

19.1 ± 0.16

tbp

22.1 ± 0.24

22.6 ± 0.25

21.9 ± 0.19

21.7 ± 0.17

24.1 ± 0.26

22.2 ± 0.29

24.0 ± 0.16

23.7 ± 0.08

24.6 ± 0.19

20.6 ± 0.16

20.2 ± 0.03

16.5 ± 0.15

b2m

17.6 ± 0.05

16.9 ± 0.03

18.2 ± 0.16

17.1 ± 0.01

15.9 ± 0.14

15.8 ± 0.04

16.1 ± 0.01

16.7 ± 0.01

17.4 ± 0.07

16.7 ± 0.29

16.0 ± 0.06

18.4 ± 0.22

elfa

15.2 ± 0.08

15.3 ± 0.02

15.7 ± 0.02

15.7 ± 0.27

14.8 ± 0.05

14.9 ± 0.18

14.3 ± 0.08

14.2 ± 0.04

16.7 ± 0.11

16.0 ± 0.04

14.6 ± 0.09

14.1 ± 0.03

18s

16.6± 0.07

16.6 ± 0.08

16.7 ± 0.05

16.9 ± 0.05

17.6 ± 0.13

17.0 ± 0.06

16.5 ± 0.08

16.4 ± 0.11

17.3 ± 0.10

16.8 ± 0.08

16.6 ± 0.07

17.0 ± 0.34

  1. Values represent Ct values (mean +/- SEM) of tissues of each type pooled by sex (3 independent pools per tissue type/sex/5 fish per pool). For details, see Methods and Results. Boldface indicates significant difference between male and female of the same tissue by t-test p < .05. All genes showed significant differences across tissue types by one-way ANOVA p < .05.