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Table 2 Housekeeping gene expression in different tissues of adult male (M) and female (F) zebrafish, as measured by QPCR

From: Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment

Ct Values +/- S.E.M.
  EYE BRAIN HEART LIVER MUSCLE GONAD
GENE M F M F M F M F M F M F
bactin1 16.2 ± 0.45 16.4 ± 0.26 16.4 ± 0.33 16.4 ± 0.46 17.5 ± 0.33 16.7 ± 0.57 18.2 ± 0.42 17.7 ± 0.30 17.7 ± 0.58 16.8 ± 0.51 16.2 ± 0.29 14.2 ± 0.24
tuba1 23.2 ± 0.19 23.1 ± 0.17 22.2 ± 0.23 22.3 ± 0.18 22.3 ± 0.29 23.5 ± 0.43 27.8 ± 0.28 29.2 ± 0.27 27.0 ± 0.40 25.4 ± 0.18 21.2 ± 0.30 21.4 ± 0.85
gapdh 18.4 ± 0.33 17.8 ± 0.58 22.6 ± 0.98 23.6 ± 0.84 15.0 ± 0.35 15.2 ± 0.53 15.8 ± 0.62 16.8 ± 0.62 15.4 ± 0.07 14.8 ± 0.34 20.1 ± 0.78 15.0 ± 0.45
g6pd 21.2 ± 0.08 21.6 ± 0.17 21.8 ± 0.11 22.0 ± 0.38 22.2 ± 0.07 21.4 ± 0.19 20.3 ± 0.20 21.0 ± 0.12 25.1 ± 0.37 23.3 ± 0.11 20.4 ± 0.10 19.1 ± 0.16
tbp 22.1 ± 0.24 22.6 ± 0.25 21.9 ± 0.19 21.7 ± 0.17 24.1 ± 0.26 22.2 ± 0.29 24.0 ± 0.16 23.7 ± 0.08 24.6 ± 0.19 20.6 ± 0.16 20.2 ± 0.03 16.5 ± 0.15
b2m 17.6 ± 0.05 16.9 ± 0.03 18.2 ± 0.16 17.1 ± 0.01 15.9 ± 0.14 15.8 ± 0.04 16.1 ± 0.01 16.7 ± 0.01 17.4 ± 0.07 16.7 ± 0.29 16.0 ± 0.06 18.4 ± 0.22
elfa 15.2 ± 0.08 15.3 ± 0.02 15.7 ± 0.02 15.7 ± 0.27 14.8 ± 0.05 14.9 ± 0.18 14.3 ± 0.08 14.2 ± 0.04 16.7 ± 0.11 16.0 ± 0.04 14.6 ± 0.09 14.1 ± 0.03
18s 16.6± 0.07 16.6 ± 0.08 16.7 ± 0.05 16.9 ± 0.05 17.6 ± 0.13 17.0 ± 0.06 16.5 ± 0.08 16.4 ± 0.11 17.3 ± 0.10 16.8 ± 0.08 16.6 ± 0.07 17.0 ± 0.34
  1. Values represent Ct values (mean +/- SEM) of tissues of each type pooled by sex (3 independent pools per tissue type/sex/5 fish per pool). For details, see Methods and Results. Boldface indicates significant difference between male and female of the same tissue by t-test p < .05. All genes showed significant differences across tissue types by one-way ANOVA p < .05.