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Figure 3 | BMC Molecular Biology

Figure 3

From: Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment

Figure 3

Effects of normalization with different reference genes on expression of (A) cyp19a1b and (B) cyp1a in zebrafish embryos following exposure to ICI 180,172 (10 μM) and TCDD (1 nM), respectively. The results are represented as fold-change (mean ± SEM) compared to the respective DMSO treated controls. cyp19a1b (mean Ct value: DMSO = 27.3 ± 0.07; ICI = 28.7 ± 0.02) and cyp1a (mean Ct value: DMSO = 25.7 ± 0.05; TCDD = 15.7 ± 0.01) were measured by QPCR as described in Methods. Treatment groups are those described in Methods and Results (Table 3; 50 embryos/pool; 3 biological replicates).

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