Table 2 Saccharomyces cerevisiae strains used in this study
From: Control of replication initiation by the Sum1/Rfm1/Hst1 histone deacetylase
Strain | Genotype |
|---|---|
AEY2 | MAT a can1-100 ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 (W303-1A) |
AEY1499 | AEY2 hst1Δ::KanMX |
AEY3358 | AEY2 sum1Δ::HisMX |
AEY3973 | AEY2, but hht1-hhf1Δ::LEU2 hht2-hhf2Δ::HIS3, lys2Δ::hisG + pCEN4- TRP1 HHF1-HHT1 |
AEY3974 | AEY2, but hht1-hhf1Δ ::LEU2 hht2-hhf2Δ::HIS3, lys2Δ::hisG + pCEN4- TRP1 hhf1-10(H4K5,8,12,16Q) HHT1 |
AEY3940 | AEY2, but MAT α orc2-1, rfm1Δ::KanMX + pRS316-ORC2 |
AEY4140 | AEY2, but orc2-1, hst1Δ ::KanMX + pRS315-ORC2 + pRS316-ORC2 |