Figure 4
From: Control of replication initiation by the Sum1/Rfm1/Hst1 histone deacetylase
Mutation of lysine to glutamine in the N-terminal tail of histone H4 caused a similar initiation defect as sum1Δ and hst1Δ. A wild-type (AEY3973) and a mutant strain (AEY3974) with K5, 8, 12 and 16 of histone H4 mutated to glutamine carrying CEN4-URA3 plasmids with ARS606 (pAE1126), ARS1013 (pAE1081) or ARSH4 (pRS316) were streaked on minimal medium lacking uracil and incubated for three days at 30°C.