sum1Δ and hst1Δ caused increased acetylation of H4 K5 at selected origins of replication. The amount of DNA from immunoprecipitated wild-type (wt, AEY2), sum1Δ (AEY3358) and hst1Δ (AEY1499) strains with anti-acetyl-histone H4 antibodies is shown relative to the input DNA. Quantitative qPCR was performed for eight selected origins and two controls (ARSH4 and CDC20). Error bars represent the average of six samples from two independent experiments. A- anti-acetyl-histone H4 K5, B- anti-acetyl-histone H4 K12, C- anti-acetyl-histone H4 K16, D- anti-acetyl-histone H4.