Alignment analysis of GFG homologues. Alignment of five GFG homologues was performed with ClustalW. Amino acid identity across all species is indicated by asterisks (*) below the sequence. The Nudix motif (GxxxxxExxxxxxxREuxEExGu) is indicated and amino acid identities within the motif are highlighted. The predicted mitochondrial targeting signal peptides (MTS) are boxed. The putative cleavage sites of mitochondrial signal peptidase are located after the last amino acid of the predicted MTSs. Note: hu = Homo sapiens (human); ra = Rattus norvegicus (rat); Tr = Takifugu rubripes; dr = Drosophila melanogaster (fruitfly).