Alternative splice variants of the FGF antisense gene and encoded GFG protein isoforms. (A) Alternative mRNA splicing of GFG mRNA transcripts. The dashed lines indicate the regions of complementarity with exon 3 of the FGF2 gene on the opposite DNA strand. Inset: RT-PCR detection of alternative splice variants. (B) The predicted translation products of the alternatively spliced GFG mRNAs, aligned against the common nudix motif. The black line indicates the region detected by the anti-GFG antiserum (C) Western blot detection of 35 kDa and 28 kDa GFG isoforms in rat liver homogenate.