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Figure 7 | BMC Molecular Biology

Figure 7

From: The Varicella-Zoster Virus Immediate-Early 63 protein affects chromatin controlled gene transcription in a cell-type dependent manner

Figure 7

HDAC3, NF-κB recruitment, and Histone H3 acetylation on selected cellular genes in HeLa cells expressing IE63 versus control cells. (A, and B) HeLa cells were treated for one hour with TNFα (200 U/mL) and ChIP assay analysis was performed using an antibody specific of the K9 acetylated form of the histone H3 (A) and the histone deacetylase HDAC3 (B). An unspecific antibody (Flag) was used for evaluating specificity. (C) HeLa cells were treated for increasing times (from 0 to 2 h) with TNFα at a final concentration of 200 U/mL. ChIP assay using p65 antibodies was performed on total cell lysates. Real-time PCR amplification of a 100 bp fragment from the IL-8, ICAM-1 and IκBα promoter encompassing the proximal NF-κB site was carried out. ρ-values were calculated using the graphpad quickcalcs software [59] : *, significantly different from control (Inv ; p-value < 0.05) ; **, not significantly different from control (Inv ; p-value ≥ 0.05).

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