Figure 4

Relative expression of selected cellular genes in MeWo-IE63 cells versus control cells. (A) Cells were lysed in radioimmunoprecipitation assay buffer and used for immunoblotting with mouse monoclonal antibody to the ORF63 protein or with rabbit polyclonal antibody to the GFP. (B) mRNA levels (TRAF1, HSPA6, MCP-1, and HLA-DRB1) were determined by quantitative real-time PCR and normalized using the β2-microglobuline transcripts. Experiments were done at least in triplicate. Differences (n-fold) between samples were calculated using the standard-curve method and the 2^-ΔCt method. ρ-values were calculated using the graphpad quickcalcs software [59]: *, significantly different from control (Inv ; p-value < 0.05).