Figure 2

Relative expression of selected cellular genes in HeLa-IE63, HeLa-IE63-S224/T222A, HeLai-IE63-Full versus control cells (HeLa-Inv). mRNA levels (A : RCE1, C3, RAB8B, and GATA3 ; B : CYFIP2, GPR37, WIF1, and NELL2) were determined by quantitative real-time PCR and normalized using the β2-microglobuline transcripts. Experiments were done at least in triplicate. Differences (n-fold) between samples were calculated using the standard-curve method and the 2^-ΔCt method. ρ-values were calculated using the graphpad quickcalcs software [59] : *, significantly different from control (Inv ; p-value < 0.05) ; **, not significantly different from control (Inv ; p-value ≥ 0.05).