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Table 1 DNA extraction protocols

From: Extensive ssDNA end formation at DNA double-strand breaks in non-homologous end-joining deficient cells during the S phase

Name

Conditions

Warm

ESPa 50°C 18 h

Cold

ESP 0°C 18 h + HSb 0°C 18 h

  1. a ESP: 0.5 M EDTA, 2% N-lauroylsarcosine, 1 mg/ml Proteinase K, pH 8.0
  2. b HS: 1.85 M NaCl, 0.15 M KCl, 5 mM MgCl2, 2 mM EDTA, 4 mM Tris, 0.5% TritonX-100, pH 7.5
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BMC Molecular Biology

ISSN: 1471-2199

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